Blog
Insights on flow cytometry analysis, AI-assisted gating, and reproducibility in research.
How to Analyze FCS Files Without FlowJo: Free Tools, Programmatic Options, and What You Give Up
Practical guide to analyzing FCS files without a FlowJo license. Covers the FCS file format structure, free tools (FCS Express, Floreada, FCSalyzer), programmatic options (R flowCore, Python FlowKit), and what you actually give up when switching from FlowJo.
Flow Cytometry Data Analysis Software Comparison: A Decision Framework for Research, Clinical, and GxP Labs
Practical flow cytometry data analysis software comparison for 2025. Decision tree for research, clinical, and GxP labs comparing FlowJo, FCS Express, Kaluza, OMIQ, and R/Bioconductor by regulatory compliance, workflow fit, and total cost of ownership.
How to Compensate for Spectral Overlap in Flow Cytometry: Controls, Matrix Math, and Validation
Step-by-step guide to compensating for spectral overlap in flow cytometry. Includes a worked spillover matrix example with real coefficients, single-color control setup, compensation validation checks, and troubleshooting common errors.
AI-Assisted Gating in Flow Cytometry: What Is Real and What Is Hype
When does AI-assisted gating actually help in flow cytometry? Decision framework for automated gating approaches, accuracy benchmarks from recent studies, and what you need in place before adopting ML-based gating.
How to Set Up a Gating Strategy in Flow Cytometry: Sequential Gates, Back-Gating, and Reproducibility
Step-by-step guide to building a reproducible flow cytometry gating strategy. Covers time gates, scatter gating, doublet discrimination, FMO controls, back-gating validation, and troubleshooting common gating errors.
FlowJo Alternatives: What Core Facilities Are Actually Switching To
Comparing free and paid FlowJo alternatives for flow cytometry analysis. Feature matrix, switching criteria, and what actually matters for core facilities evaluating new software.